Myc Tag Antibody A-21281 from Thermo Fisher Scientific

Product Specs
ItemMyc Tag Antibody
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberA-21281
Quantity100 µL
ApplicationsImmunocytochemistry, Immunohistochemistry, Western Blot
Conjugate/TagUnconjugated
FormatPBS with 0.1% sodium azide; pH 7.2
ReactivityTag
TargetMyc Tag
IsotypeIgY
HostChicken
ImmunogenWas raised against the peptide EQKLISEEDL
ClonalityPolyclonal
Concentration1 mg/mL
Storage Temperature4° C
Antibody TypePrimary
Storage4° C

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) An engineered interleukin‐11 decoy cytokine inhibits receptor signaling and proliferation in lung adenocarcinomaBioengineering & Translational MedicineNovember 1, 2023Brianna J. McIntosh, Griffin G. Hartmann, Sean A. Yamada‐Hunter, Phillip Liu, Camille F. Williams, Julien Sage, Jennifer R. Cochranwere washed with BPBS, pelleted, and resuspended in BPBS with a 1:5000 dilution of chicken anti‐c‐myc (A21281, Invitrogen) for 30 min at 4°C. Yeast were washed, pelleted, and resuspended with 1:500 dilutions of

(2) CD98hc is a target for brain delivery of biotherapeuticsNat CommunAugust 19, 2023Kylie S. Chew, Robert C. Wells, Arash Moshkforoush, Darren Chan, Kendra J. Lechtenberg, Hai L. Tran, Johann Chow, Do Jin Kim, Yaneth Robles-Colmenares, Devendra B. Srivastava, et al.similarly as described12. Briefly, libraries were labeled with chicken anti-cMyc (1:1000, Invitrogen A-21281) and anti-chicken Alexa Fluor 488 (1:1000, Invitrogen A-11039) to assess library expression on the surface

(3) Structural and functional map for forelimb movement phases between cortex and medullaCellJanuary 5, 2023Wuzhou Yang, Harsh Kanodia, Silvia ArberRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, chicken anti-GFP,Invitrogen,Cat# A10262;RRID: AB_2534023 chicken anti-Myc,Invitrogen,Cat# A21281;RRID: AB_2535826 Rabbit anti-GFAP,Millipore,Cat# AB5804;RRID: AB_2109645 goat anti-ChAT,Millipore,Cat#

(4) ZEITLUPE Promotes ABA-Induced Stomatal Closure in Arabidopsis and PopulusFrontiers in Plant ScienceMarch 2, 2022Manuela Jurca et al.β-mercaptoethanol, 2% SDS and 62.5 mM TRIS pH 6.8) and incubated with anti-c-Myc chicken antibody (A21281; Thermo Fisher Scientific) to confirm the presence of Myc-tagged proteins in the complex. Co-IP results,

(5) Naive human B cells engage the receptor binding domain of SARS-CoV-2, variants of concern, and related sarbecovirusesScience immunologyDecember 10, 2021Feldman J, Bals J, Altomare CG, St Denis K, Lam EC, Hauser BM, Ronsard L, Sangesland M, Moreno TB, Okonkwo V, et al.antigen binding (RBD-Fc APC tetramers) and scFv expression (chicken anti-c-myc IgY; Invitrogen, cat. no A-21281). Following two washes in PBS with 0.1% w/v BSA, yeast was stained with donkey anti-chicken IgY AF488

(6) Memory B cell repertoire for recognition of evolving SARS-CoV-2 spikeCellSeptember 16, 2021Tong P, Gautam A, Windsor IW, Travers M, Chen Y, Garcia N, Whiteman NB, McKay LGA, Storm N, Malsick LE, et al.48 h to induce expression. RBD and NTD were detected by anti-c-Myc IgY antibody (ThermoFisher, Cat. A21281). Yeast expressing RBD or NTD were incubated with antibody supernatant and anti-c-Myc IgY on ice for

(7) Functional diversity for body actions in the mesencephalic locomotor regionCellAugust 19, 2021Ferreira-Pinto MJ, Kanodia H, Falasconi A, Sigrist M, Esposito MS, Arber S.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, chicken anti-GFP,Invitrogen,Cat# A10262; RRID: AB_2534023 chicken anti-Myc,Invitrogen,Cat# A21281; RRID: AB_2535826 chicken anti-TH,Neuromics,Cat# CH23006; RRID: AB_2201403 goat anti-ChAT,Millipore,Cat#

(8) Generation of recombinant hyperimmune globulins from diverse B-cell repertoiresNature biotechnologyAugust 1, 2021Keating SM, Mizrahi RA, Adams MS, Asensio MA, Benzie E, Carter KP, Chiang Y, Edgar RC, Gautam BK, Gras A, et al.C-terminal myc tag to identify scFv expression with 1 μL per sample of undiluted anti-myc primary (A21281; Thermo Fisher Scientific, Waltham, MA, USA) and 1 μL per sample of undiluted AF488 secondary antibody

(9) Selection of a picomolar antibody that targets CXCR2-mediated neutrophil activation and alleviates EAE symptomsNature communicationsMay 5, 2021Shi X, Wan Y, Wang N, Xiang J, Wang T, Yang X, Wang J, Dong X, Dong L, Yan L, et al.BSA in PBS with 2 mM EDTA), pepN48 and anti-c-myc chicken IgY polyclonal antibody (1:500 dilution; #A21281, Invitrogen) were added to the cell suspension and incubated for 30 min at room temperature. Cells were

(10) An engineered ligand trap inhibits leukemia inhibitory factor as pancreatic cancer treatment strategyCommunications biologyApril 12, 2021Hunter SA, McIntosh BJ, Shi Y, Sperberg RAP, Funatogawa C, Labanieh L, Soon E, Wastyk HC, Mehta N, Carter C, et al.cells were washed once with BPBS, then incubated with a 1:5000 dilution of chicken anti-c-myc antibody (A21281, Invitrogen). For His6-tagged proteins, a 1:500 dilution of rabbit anti-6-His-FITC antibody (NC0585792,

(11) Alternative splicing of the metalloprotease ADAMTS17 spacer regulates secretion and modulates autoproteolytic activityFASEB journal : official publication of the Federation of American Societies for Experimental BiologyFebruary 1, 2021Balic Z, Misra S, Willard B, Reinhardt DP, Apte SS, Hubmacher D.incubation with the monoclonal ADAMTS17 clone 3B7 (1:500) or the polyclonal anti-Myc antibody (1:500, A21281, Invitrogen), diluted in 10% normal goat serum in PBS for 2 h at RT. Cells were rinsed 3 × 5 minutes

(12) Genetically encoded live-cell sensor for tyrosinated microtubulesThe Journal of cell biologyOctober 5, 2020Kesarwani S, Lama P, Chandra A, Reddy PP, Jijumon AS, Bodakuntla S, Rao BM, Janke C, Das R, Sirajuddin M.with biotin-TUBA1A 440–451 peptide (100 µM) and chicken anti-c-Myc antibody (Invitrogen; catalog no. A21281) in 1:250 dilution at room temperature for 1 h. Cells were pelleted, washed twice with PBS-BSA, and

(13) An engineered antibody binds a distinct epitope and is a potent inhibitor of murine and human VISTAScientific reportsSeptember 16, 2020Mehta N, Maddineni S, Kelly RL, Lee RB, Hunter SA, Silberstein JL, Parra Sperberg RA, Miller CL, Rabe A, Labanieh L, et al.500 nM; Sort 1.3, 50 nM; and sort 1.4, 5 nM). A 1:5,000 dilution of chicken anti-c-Myc (Invitrogen, A21281) was added with antigen in each sort round. Yeast were then washed and labeled on ice with 1:250 dilution

(14) Small Molecule Dysregulation of TEAD Lipidation Induces a Dominant-Negative Inhibition of Hippo Pathway SignalingCell reportsJune 23, 2020Holden JK, Crawford JJ, Noland CL, Schmidt S, Zbieg JR, Lacap JA, Zang R, Miller GM, Zhang Y, Beroza P, et al.

(15) Engineering a potent receptor superagonist or antagonist from a novel IL-6 family cytokine ligandProceedings of the National Academy of Sciences of the United States of AmericaJune 23, 2020Kim JW, Marquez CP, Sperberg RAP, Wu J, Bae WG, Huang PS, Sweet-Cordero EA, Cochran JR.Cell Signaling Technology. Anti–β-tubulin antibody was from Covance (MMS-410P). Chicken anti-c-Myc antibody (A21281) and phospho(Tyr759)-gp130 antibody (PA564830) were purchased from Thermo Scientific and anti-chicken

(16) The Cellular Protein CAD is Recruited into Ebola Virus Inclusion Bodies by the Nucleoprotein NP to Facilitate Genome Replication and TranscriptionCellsMay 1, 2020Brandt J, Wendt L, Bodmer BS, Mettenleiter TC, Hoenen T.[F1804], and the anti-c-myc antibody used for IFA analysis was obtained from Thermo Fisher Scientific [A-21281]. Primary antibodies against NP (rabbit anti-EBOV NP polyclonal antibody), GAPDH (mouse anti-GAPDH clone

(17) Affinity maturation of antibodies by combinatorial codon mutagenesis versus error-prone PCRmAbsJanuary 1, 2020Simons JF, Lim YW, Carter KP, Wagner EK, Wayham N, Adler AS, Johnson DS.incubated for 30 min at room temperature in the presence of chicken anti-c-myc polyclonal antibody (A21281, Thermo Fisher Scientific, Waltham, MA) at 5 μg/ml and the appropriate biotinylated antigen. For the

(18) Yeast surface display of full-length human microtubule-associated protein tauBiotechnology progressJanuary 1, 2020Wang S, Cho YK.No. 132500, 1:100 dilution) or anti-c-Myc tag chicken polyclonal antibody (Thermo Fisher, Cat. No. A21281, 1:250 dilution) was used to detect the c-Myc epitope tag at the Cterminus of tau. After the primary

(19) Engineering of a novel subnanomolar affinity fibronectin III domain binder targeting human programmed death-ligand 1Protein engineering, design & selection : PEDSDecember 31, 2019Ramakrishnan S, Natarajan A, Chan CT, Panesar PS, Gambhir SS.affinity maturation studies, yeast cells in single-cell suspension were stained with chicken anti-Myc (A21281, Thermo Fisher Scientific, Waltham, MA) and goat anti-chicken Alexa Fluor 555 ® ® 647 conjugate (S21371),

(20) Antitumor activity of an engineered decoy receptor targeting CLCF1-CNTFR signaling in lung adenocarcinomaNature medicineNovember 1, 2019Kim JW, Marquez CP, Kostyrko K, Koehne AL, Marini K, Simpson DR, Lee AG, Leung SG, Sayles LC, Shrager J, et al.CLCF1, yeast were pelleted, washed and resuspended in PBSA with 1:500 ratio of chicken anti-c-Myc (#A21281, Invitrogen) for 30 min at 4°C. Yeast were then washed and pelleted, and secondary labeling was performed

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Myc Tag Antibody from Thermo Fisher Scientific

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Myc Tag Antibody from Thermo Fisher Scientific

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